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Volume 67, Issue 2
  • ISSN: 2056-5135
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  • oa Screening of Bioactive Compounds for Biomedical and Industrial Uses from Isolated from the Parsık Cave, Turkey

    Identifying novel compounds from extreme environments

  • Authors: Nahdhoit Ahamada Rachid1 and Nihal Doğruöz Güngör2
  • Affiliations: 1 Institute of Graduate Studies in Sciences, Istanbul UniversityBalabanaga Mah. Sehzadebasi Cd., 34134 Vezneciler, Fatih-IstanbulTurkey 2 Department of Biology, Faculty of Science, Istanbul University34134 Vezneciler, IstanbulTurkey
  • Source: Johnson Matthey Technology Review, Volume 67, Issue 2, Apr 2023, p. 159 - 170
  • DOI: https://doi.org/10.1595/205651322X16482034395036
    • Received: 03 Jan 2022
    • Accepted: 24 Mar 2022
    • Published online: 25 Mar 2022

Abstract

The need to avoid health issues and pollution of the environment from the use of chemicals and synthetic materials inspires scientists to search for new biological compounds beneficial to human beings. Caves, being extreme environments, might be potential sources of these compounds. , one of the main groups that colonise these environments, are known to generate natural bioactive compounds. To investigate the potential uses of Parsık Cave , identification of this group of isolates and the investigation of their secreted biological compounds constituted the principal aim of the present study. The identification was achieved by sequencing 16S rRNA genes of 41 selected bacteria of which 28 species were identified as . (21%) and (14%) were the most identified genera. Antimicrobial effects of the isolates P1 and P16 were observed against standard microorganisms like . The gas chromatography-mass spectrometry (GC-MS) analysis of their broth showed compounds with known antimicrobial, antioxidant or anticancer properties as well as unknown compounds. Polyketide synthase (PKS) and non-ribosomal peptide synthases (NRPS) respectively were amplified in 32.1% and 53.5% of the identified while 25% were found to have both NRPS and PKS amplified. Amylase, gelatinase, cellulase, deoxyribonuclease (DNase), urease and casein hydrolysing activities were observed in the identified . These results show that from Parsık Cave might be good sources of industrial and biotechnological compounds. Furthermore, discovery of new bioactive compounds from these bacteria is promising due to the many unknown compounds observed in the GC-MS analysis and the high percentage of NRPS and PKS gene amplification.

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2022-03-25
2024-12-26
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References

  1. D. Bukelskis, D. Dabkeviciene, L. Lukoseviciute, A. Bucelis, I. Kriaučiūnas, J. Lebedeva, N. Kuisiene, Front. Microbiol., 2019, 10, 2149 LINK https://doi.org/10.3389/fmicb.2019.02149 [Google Scholar]
  2. S. Zada, W. Sajjad, M. Rafiq, S. Ali, Z. Hu, H. Wang, R. Cai, Microb. Ecol., 2021, 84, (3), 676 LINK https://doi.org/10.1007/s00248-021-01889-3 [Google Scholar]
  3. Z. Cyske, W. Jaroszewicz, M. Żabińska, P. Lorenc, M. Sochocka, P. Bielańska, Ł. Grabowski, L. Gaffke, K. Pierzynowska, G. Wȩgrzyn, Acta Biochim. Polon., 2021, 68, (4), 565 LINK https://doi.org/10.18388/abp.2020_5887 [Google Scholar]
  4. M. D. Türkgenci, N. Doğruöz Güngör, Geomicrobiol. J., 2021, 38, (9), 816 LINK https://doi.org/10.1080/01490451.2021.1964110 [Google Scholar]
  5. H. A. Barton, V. Jurado, Microbe, 2007, 2, 132 LINK https://digital.csic.es/handle/10261/61951 [Google Scholar]
  6. N. Cheeptham, ‘Advances and Challenges in Studying Cave Microbial Diversity’, in “Cave Microbiomes: A Novel Resource for Drug Discovery”, ed. N. Cheeptham, Springer Science+Business Media, New York, USA, 2013, pp. 1–34 LINK https://doi.org/10.1007/978-1-4614-5206-5_1 [Google Scholar]
  7. S. Castanier, G. Le Méteyer-Levrel, J.-P. Perthuisot, S. M. Awramik, ‘Bacterial Roles in the Precipitation of Carbonate Minerals’, in “Microbial Sediments”, eds. R. E. Riding, Springer-Verlag, Berlin, Germany, 2000, pp. 32–39 LINK https://doi.org/10.1007/978-3-662-04036-2_5 [Google Scholar]
  8. H. A. Barton, D. E. Northup, J. Cave KarstStud., 2007, 69, (1), 163 [Google Scholar]
  9. I. Tomova, I. Lazarkevich, A. Tomova, M. Kambourova, E. Vasileva-Tonkova, Int. J. Speleol., 2013, 42, (1), 65 LINK https://doi.org/10.5038/1827-806x.42.1.8 [Google Scholar]
  10. K. Tomczyk-Żak, U. Zielenkiewicz, Geomicrobiol. J., 2016, 33, (1), 20 LINK https://doi.org/10.1080/01490451.2014.1003341 [Google Scholar]
  11. B. Çandiroğlu, N. Doğruöz Güngör, Johnson Matthey Technol. Rev., 2020, 64, (4), 466 LINK https://technology.matthey.com/article/64/4/466-479/ [Google Scholar]
  12. P. Rangseekaew, W. Pathom-aree, Front. Microbiol., 2019, 10, 387 LINK https://doi.org/10.3389/fmicb.2019.00387 [Google Scholar]
  13. S. Zada, J. Xie, M. Yang, X. Yang, W. Sajjad, M. Rafiq, F. Hasan, Z. Hu, H. Wang, Appl. Microbiol. Biotechnol., 2021, 105, (23), 8921 LINK https://doi.org/10.1007/s00253-021-11658-4 [Google Scholar]
  14. N. Nawani, B. Aigle, A. Mandal, M. Bodas, S. Ghorbel, D. Prakash, BioMed Res. Int., 2013, 687190 LINK https://doi.org/10.1155/2013/687190 [Google Scholar]
  15. D. V. Axenov-Gibanov, I. V. Voytsekhovskaya, B. T. Tokovenko, E. S. Protasov, S. V. Gamaiunov, Y. V. Rebets, A. N. Luzhetskyy, M. A. Timofeyev, PLoS One, 2016, 11, (2), e0149216 LINK https://doi.org/10.1371/journal.pone.0149216 [Google Scholar]
  16. J. W.-F. Law, V. Letchumanan, L. T.-H. Tan, H.-L. Ser, B.-H. Goh, L.-H. Lee, Prog. Microbe. Mol. Biol., 2020, 3, (1), a0000064 LINK https://doi.org/10.36877/pmmb.a0000064 [Google Scholar]
  17. “Biotechnology of Antibiotics”, ed. W. R. Strohl, 2nd Edn., CRC Press, Boca Raton, USA, 1997, 860 pp LINK https://doi.org/10.1201/9780367804558 [Google Scholar]
  18. D. Prakash, N. Nawani, M. Prakash, M. Bodas, A. Mandal, M. Khetmalas, B. Kapadnis, BioMed Res. Int., 2013, 264020 LINK https://doi.org/10.1155/2013/264020 [Google Scholar]
  19. M. Maciejewska, D. Adam, A. Naômé, L. Martinet, E. Tenconi, M. Całusińska, P. Delfosse, M. Hanikenne, D. Baurain, P. Compère, M. Carnol, H. A. Barton, S. Rigali, Front. Microbiol., 2017, 8, 1181 LINK https://doi.org/10.3389/fmicb.2017.01181 [Google Scholar]
  20. S. Ghosh, N. Kuisiene, N. Cheeptham, Biochem. Pharmacol., 2017, 134, 18 LINK https://doi.org/10.1016/j.bcp.2016.11.018 [Google Scholar]
  21. D. H. Amin, N. A. Abdallah, A. Abolmaaty, S. Tolba, E. M. H. Wellington, Bull. Natl. Res. Cent., 2020, 44, 5 LINK https://doi.org/10.1186/s42269-019-0266-8 [Google Scholar]
  22. S. Jiang, W. Sun, M. Chen, S. Dai, L. Zhang, Y. Liu, K. J. Lee, X. Li, Antonie Van Leeuwenhoek, 2007, 92, (4), 405 LINK https://doi.org/10.1007/s10482-007-9169-z [Google Scholar]
  23. G. C. A. Amos, C. Borsetto, P. Laskaris, M. Krsek, A. E. Berry, K. K. Newsham, L. Calvo-Bado, D. A. Pearce, C. Vallin, E. M. H. Wellington, PLoS One, 2015, 10, (9), e0138327 LINK https://doi.org/10.1371/journal.pone.0138327 [Google Scholar]
  24. M. S. Kuyukina, I. B. Ivshina, ‘Application of Rhodococcus in Bioremediation of Contaminated Environments’, in “Biology of Rhodococcus”, ed. H. M. Alvarez, Microbiology Monographs, Vol. 16, Springer-Verlag, Berlin, Germany, 2010, pp. 231–262 LINK https://doi.org/10.1007/978-3-642-12937-7_9 [Google Scholar]
  25. E. M. Rodrigues, K. H. M. Kalks, M. R. Tótola, J. Environ. Manage., 2015, 156, 15 LINK https://doi.org/10.1016/j.jenvman.2015.03.016 [Google Scholar]
  26. A. R. Johnson, E. E. Carlson, J. Am. Soc. Mass Spectrom., 2019, 30, (8), 1464 LINK https://doi.org/10.1007/s13361-019-02210-w [Google Scholar]
  27. C. Vézina, C. Bolduc, A. Kudelsk, P. Audet, Antimicrob. Agents Chemother., 1979, 15, (5), 738 LINK https://doi.org/10.1128/aac.15.5.738 [Google Scholar]
  28. S. A. Waksman, H. A. Lechevalier, Science, 1949, 109, (2830), 305 LINK https://doi.org/10.1126/science.109.2830.305 [Google Scholar]
  29. P. Pusparajah, V. Letchumanan, J.W.-F. Law, N.-S. Ab Mutalib, Y. S. Ong, B.-H. Goh, L. T.-H. Tan, L.-H. Lee, Int. J. Mol. Sci., 2021, 22, (17), 9360 LINK https://doi.org/10.3390/ijms22179360 [Google Scholar]
  30. W. Jaroszewicz, P. Bielańska, D. Lubomska, K. Kosznik-Kwaśnicka, P. Golec, Ł. Grabowski, E. Wieczerzak, W. Dróżdż, L. Gaffke, K. Pierzynowska, G. Węgrzyn, A. Węgrzyn, Antibiotics, 2021, 10, (10), 1212 LINK https://doi.org/10.3390/antibiotics10101212 [Google Scholar]
  31. F. Z. Djebbah, L. Belyagoubi, D. E. Abdelouahid, F. Kherbouche, N. A. Al-Dhabi, M. V. Arasu, B. Ravindran, J. Infect. Public Health, 2021, 14, (11), 1671 LINK https://doi.org/10.1016/j.jiph.2021.09.019 [Google Scholar]
  32. G. A. Quinn, A. M. Banat, A. M. Abdelhameed, I. M. Banat, J. Med. Microbiol., 2020, 69, (8), 1040 LINK https://doi.org/10.1099/jmm.0.001232 [Google Scholar]
  33. P. Sharma, B. Choudhary, A. Nagpure, R. K. Gupta, J. Environ. Biol., 2016, 37, (6), 1231 LINK https://doi.org/10.22438/jeb/37/6/mrn.1000 [Google Scholar]
  34. P. H. K. Ngai, T. B. Ng, Peptides, 2004, 25, (12), 2063 LINK https://doi.org/10.1016/j.peptides.2004.08.003 [Google Scholar]
  35. S. Sanchez, R. Rodríguez-Sanoja, A. Ramos, A. L. Demain, J. Antibiot., 2018, 71, (1), 26 LINK https://doi.org/10.1038/ja.2017.142 [Google Scholar]
  36. R. Salwan, V. Sharma, ‘’The Role of Actinobacteria in the Production of Industrial Enzymes’, in “New and Future Developments in Microbial Biotechnology and Bioengineering: Actinobacteria: Diversity and Biotechnological Applications”, eds. B. P. Singh, V. K. Gupta, A. K. Passari, Elsevier BV, Amsterdam, The Netherlands, 2018, pp. 165–177 LINK https://doi.org/10.1016/b978-0-444-63994-3.00011-4 [Google Scholar]
  37. F. J. Contesini, R. R. de Melo, H. H. Sato, Crit. Rev. Biotechnol., 2018, 38, (3), 321 LINK https://doi.org/10.1080/07388551.2017.1354354 [Google Scholar]
  38. A. K. Sharma, V. Sharma, J. Saxena, B. Yadav, A. Alam, A. Prakash, Int. J. Sci. Res. Environ. Sci., 2015, 3, (9), 0334 [Google Scholar]
  39. A. Razzaq, S. Shamsi, A. Ali, Q. Ali, M. Sajjad, A. Malik, M. Ashraf, Front. Bioeng. Biotechnol., 2019, 7, 110 LINK https://doi.org/10.3389/fbioe.2019.00110 [Google Scholar]
  40. M. Singh, Curr. Sci., 2019, 116, (11), 1840 LINK https://doi.org/10.18520/cs/v116/i11/1840-1849 [Google Scholar]
  41. J. Hamedi, M. Kafshnouchi, M. Ranjbaran, Saudi J. Biol. Sci., 2019, 26, (7), 1587 LINK https://doi.org/10.1016/j.sjbs.2018.10.010 [Google Scholar]
  42. H. L. Brown, M. Reuter, K. Hanman, R. P. Betts, A. H. M. van Vliet, PLoS One, 2015, 10, (3), e0121680 LINK https://doi.org/10.1371/journal.pone.0121680 [Google Scholar]
  43. É. B. de Melo Riceto, R. de Paula Menezes, M. P. A. Penatti, R. dos Santos Pedroso, Rev. Iberoam. Micol., 2015, 32, (2), 79 LINK https://doi.org/10.1016/j.riam.2013.11.003 [Google Scholar]
  44. B. C. Behera, B. K. Sethi, R. R. Mishra, S. K. Dutta, H. N. Thatoi, J. Genet. Eng. Biotechnol., 2017, 15, (1), 197 LINK https://doi.org/10.1016/j.jgeb.2016.12.001 [Google Scholar]
  45. S. K. Gupta, S. Kataki, S. Chatterjee, R. K. Prasad, S. Datta, M. G. Vairale, S. Sharma, S. K. Dwivedi, D. K. Gupta, J. Clean. Prod., 2020, 258, 120351 LINK https://doi.org/10.1016/j.jclepro.2020.120351 [Google Scholar]
  46. N. A. Al-Dhabi, G. A. Esmail, V. Duraipandiyan, M. V. Arasu, Saudi J. Biol. Sci., 2019, 26, (4), 758 LINK https://doi.org/10.1016/j.sjbs.2019.03.009 [Google Scholar]
  47. N. A. Al-Dhabi, G. A. Esmail, A.-K. M. Ghilan, M. V. Arasu, Saudi J. Biol. Sci., 2020, 27, (1), 474 LINK https://doi.org/10.1016/j.sjbs.2019.11.011 [Google Scholar]
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Screening of Bioactive Compounds for Biomedical and Industrial Uses from Actinobacteria Isolated from the Parsık Cave, Turkey
Johnson Matthey Technology Review 67, 159 (2023); https://doi.org/10.1595/205651322X16482034395036
/content/journals/10.1595/205651322X16482034395036
/content/journals/10.1595/205651322X16482034395036
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